Reingard Grabherr

Leiterin des CD-Labors

Kontakt:
Universität für Bodenkultur, Wien,  Department für Biotechnologie
Muthgasse 18, A-1190 Wien, Österreich
Tel.: +43-1-47654-6242
Fax: +43-1-3697615
e-mail: reingard.grabherr@boku.ac.at

Curriculum vitae

Akademischer Grad: Ao. Univ. Prof. Dipl.-Ing. Dr. nat. techn.

Gebürtig: 3. November 1964 in Wien, Österreich

Staatsbürgeschaft: Österreich

Ausbildung

  • Matura: Neusprachliches Gymnasium, Sacre Coeur, Pressbaum (1982)
  • DI für Lebensmittel- und Biotechnologie, Universität für Bodenkultur, Wien (1988)
  • Doktoratsstudium, Institute für angewandte Mikrobiologie, Universität für Bodenkultur, Wien, bei Professor Hermann Katinger (1989)
  • Visiting Scientist, Department of Plant Pathology, University of Nebraska-Lincoln, NE, USA, bei Professor James L. VanEtten (1989-1991)
  • Doktorat, Universität für Bodenkultur, Wien (1992)
  • PostDoc an der Universität für Bodenkultur, Wien, Institute für angewandte Mikrobiologie bei Professor Hermann Katinger (1992-1998).
  • Assistenzprofessor an der Universität für Bodenkultur, Wien (Februar 1998)
  • Habilitation in “Angewandter Virologie” a. o. Univ. Professor an der Universität für Bodenkultur, Wien, Institute für angewandte Mikrobiologie (März 2002)
  • A.o.Univ. Professor at the University of Natural Resources and Applied Life Sciences, Vienna; Institute of Applied Microbiology (March 2002)

Preise

  • Austrian Programme for Advanced Research and Technology (APART) by the Austrian Academy of Sciences (May 1998)
  • Houska-Annerkennungspreis 2006 der BA-CA Privatstiftung für das Projekt „Neuartige Herstellung von Protein und DNA als Therapeutika“
  • Science2business Award 2007 with Boehringer Ingelheim
  • Science2business Award 2008 with Boehringer Ingelheim

Lehrtätigkeit

  • Lehrtätigkeit während eines 12-tägigen Kursese in Kocaely, Türkei: „Phage Display Technology for the Development of Antigen Binding“, (1994)
  • Vorlesung: “Angewandte Virologie” an der Universität für Bodenkultur, Wien (seit Oktober 1998)
  • Biotechnologieseminare an der Universität für Bodenkultur, Wien (seit Oktober 1998)
  • Übungen:  “Angewandte Mikrobiologie”  an der Universität für Bodenkultur, Wien (seit Oktober 2003)
  • Vorlesung: „Cell Factory“ an der Universität für Bodenkultur, Wien (seit 2005)
  • Vorlesung: „Systems Biology“ an der Universität für Bodenkultur, Wien (seit 2005)
  • Vorlesung: “Gene technology” an der Fachhochschule Wr. Neustadt, Tulln, Lehrgang Biotechnologie, (2006-2007)

Mitgliedschaften

  • Mitglied der Österreichischen Gesellschaft für Biotechnologie (ÖGBT) (1999-)
  • Vorstandsmitglied (Schriftführerin) der Österreichischen Gesellschaft für Genetik und Gentechnik (ÖGGGT) (2002-)
  • Vorstandsmitglied (Vizepräsidentin) der Österreichischen Gesellschaft für Protein Engineering (ÖGPE) (2002-)
  • Vorstandsmitglied (Präsidentin) der Österreichischen Gesellschaft für Systembiologie (ÖGSB) (2003-)
  • Boardmember of the Protein Expression International Society (PEIS) (2005-)
  • Internes Mitglied des Komitees für biologische Sicherheit am Institut für Angewandte Mikrobiologie, Univ. f. Bodenkultur, Wien (2000-)
  • Externes Mitglied des Komitees für biologische Sicherheit der Fa. Igeneon GmbH, (2000-2003) Wien
  • Externes Mitglied des Komitees für biologische Sicherheit der Fa. Apeiron, Wien (2004-)
  • Mitglied der Gentechnikkomission, Bundesministerium für Gesundheit, Österreich (2008-)
  • Beauftragte für biologische Sicherheit am Department für Biotechnologie der Universität für Bodenkultur, Wien (2009-)

Mitglied im “Organizing Commitee” folgender internationaler Meetings

  • 5th Gene Delivery and Cellular Protein Conference, September 2001, Semmering, Austria
  • 6th Protein Expression in Animal Cells Conference, September 2003, Mont Tremblant, Quebec, Canada
  • BIOTECHNOLOGY International Review Conference, Polish Academy of Sciences, Scientific Centre in Vienna, November 2004, Vienna, Austria
  • 7th Protein Expression in Animal Cells Conference, September 2005, Crete, Greece
  • Gemeinsame Jahrestagung der ÖGBT, ÖGGGT, ÖGBM, ANGT, September, 2005, Vienna, Austria
  • 8th Protein Expression in Animal Cells Conference, September 2007, Angra dos Reis, Brasil
  • 9th Protein Expression in Animal Cells Conference, September 2009, Jackson Hole, Wyoming, USA
  • 10th Protein Expression in Animal Cells Conference, September 2011, Lisboa, Portugal

Projekte

Project IMMUNO-CRADA
Activation of the factor VIII promoter in a human cell line. February 1995-July 1997.
Project leader: DI Dr.Reingard Grabherr
DI Dr. Renate Kunert

FWF-Project P10273-MOB
Expression of proteins on the baculovirus surface for screening and generation of ligands in insect cells.
1.4.1994-31.3.1996
Project leader: Univ. Ass. Doz. DI Dr. Otto Doblhoff-Dier
Co-applicant: DI Dr. Reingard Grabherr
C-worker: Dipl. Ing. Dr. Wolfgang Ernst

FWF-Projekt P11523-MOB
Expression of proteins on the  surface of baculoviruses: etablishment, optimization, application.
1.8.1996-31.7.1998
Project leader: DI Dr. Reingard Grabherr
Co-applicant: Univ. Ass. Doz. Dipl. Ing. Dr. Otto Doblhoff-Dier
Co-worker: DI Dr. Wolfgang Ernst

FWF-Projekt P12760-MOB
Baculovirus Surface Display: Establishment, Optimisation and Application.
1.3.1999-31.9.2000
Project leader: DI Dr. Reingard Grabherr
Co-worker: DI Dr. Wolfgang Ernst

EU-Projekt
Baculovirus Surface Display-Development and Applications
Partner 1 (Koordinator): Prof. Dr. Christian Oker-Blom
University of Helsinki, Department of Biotechnology, Helsinki, Finland
Partner 2: Prof. Dr. Ian Jones
NERC Institute of Virology, Mansfield Road, Oxford, OX1 3SR, UK
Partner 3: DI Dr. Reingard Grabherr
Institute of Applied Microbiology, Agricultural University, Vienna

Project withGlaxo-Smith-Kline (Dr. Tom Kost)
Comparison of different mammalian promoters for expression in mammalian cells using baculovirus as transfer vehicle
1.2.2002-31.1.2003
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Dr. Alexandra Spenger

FWF-Project P15759
Rescuing of Influenza B Viruses Using Novel Baculovirus Gene Delivery Tools
1.10.2002-30.9.2004
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Dr. Lars Töllner

Project with IGENEON (DI Dr. Gottfried Himmler)
Production of EpCam and EpCam-fragments in insect cells
1.11.2002-30.10.2003
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: Ing. Stefan Gross

WWTF-Project with Boehringer-Ingelheim Austria (D. I. Hans Huber)
Design of novel, optimized plasmids for gentherapeutic applications
1.1.2003-31.12.2006
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Irene Schotola

FFF-Project with Biomin (Dr. Gerd Schatzmayer)
Development of production strains for detoxification of Fumonisin and Zearalenon.
1.10.2003-30.9.2006
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Stefan Heinl

FFG-Project with Biomin (Dr. Dieter Moll)
Enzymatische Detoxifizierung von Mycotoxinen.
1.1.2007-31.12.2007
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Dr. Stefan Heinl

FWF-Project P17361-B13
Novel Method for the Fast and Efficient Generation of Influenza Live Attenuated Vaccine: Co-Infection of Especially Designed Virus Like Particles.
1.3. 2005-31.1.2009
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-workers: DI Theresa Schinko, DI Sabine Nakowitsch, DI Florian Krammer

FFG-Project with Boehringer-Ingelheim Austria (DI Dr. Hans Huber)
Antibiotikafreie Plasmidselektion
1.1.2007-31.12.2009
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Jürgen Mairhofer

Project with Lactosan GmbH (DI Dr. Hans Lettner)
Effektive Degradation of Oligo- and Polysaccharides by Development of Genetically Engineered Lactobacillus.
1.8.2007-31.7.2010
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Dr. Stefan Heinl

FFG-Project with OnePharm (DI Dr. Oliver Szolar)
Development of an Online Monitoring System for Transcriptional Regulation in Animal Cells Using a Baculovirusvector.
1.9.2007-30.6.2009
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Theresa Schinko

FFG-Project with f-star (DI Dr. Gottfried Himmler)
Surface Display Libraries in Insect Cells and Mammalian Cells
1.11.2007- 30.6.2009
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Dieter Palmberger

Christian Doppler Laboratory for Genetically Engineered Lactic Acid Bacteria
Nov. 2008- Okt. 1015
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr

Project with SAVIRA (DI Dr. Oliver Szolar)
Development of an Activity Assay for Influenza Polymerases
1.1.2010-30.6.2010
Project leader: a.o. Univ. Prof. DI Dr. Reingard Grabherr
Co-worker: DI Dr. Florian Krammer

Vorträge

  • „Developing and Optimizing a Eukaryotic Surface Display System Using the Baculovirus Insect Cell Expression System“ International conference „Transient Gene Expression in Animal Cells“, May, 1997, Jersey, UK
  • „Baculovirus Surface Display: Development, Optimization and Future Aspects“ Phage Club Meeting, May 1999, La Grande-Motte, France
  • „Baculovirus Surface Display: A New Tool for Generating Molecular Diversity” University of Nebraska, Lincoln,October 1999, Nebraska, USA
  • „Baculovirus Surface Display“: Establishment and Applications Institut für Molekularbiologie, April 2000, Salzburg, Austria
  • „Baculovirus Surface Display“: Establishment and Applications Dechema Biotechnologie 2000,Septemper 2000, Berlin, Germany
  • “Advances and Applications in Baculovirus Surface Display Technology“  Biotechnology Research Institute, June 2002, Montreal, Canada
  • „Baculovirus transduction of mammalian cells: improvements and applications“ 3rd Conference on Protein Expression, November 2004, Algarve, Portugal
  • „Novel Influenza Virus Like Particles in Insect Cells“ Department of Chemical Engineering, University of Nebraska, Lincoln, July 2005, Nebraska, USA
  • „Alternative Approach to Antibiotic Plasmid Selection“ Biopharmaceutical Manufacturing Excellence: APIs Derived from Microorganisms by the International Association for Pharmaceutical Technology, October 2006, Vienna, Austria
  • “Expression of Influenza Virus Like Particles Using the Baculovirus System” Monolith Summer Symposium, June 2010, Porto Roz, Slovenia

Publikationen

  1. Shields S., Burbank D., Grabherr R., and VanEtten J. L. (1990). Cloning and Sequencing the Cytosine Methyltransferase Gene M.CviJI from Chlorella Virus Il-3A. Virology 176, 16-27.
  2. Nelson M., VanEtten J. L.,and Grabherr R. (1992). DNA Sequencing of Four Bases Using Three Lanes.Nucleic Acids Research 20, 1345-1348.
  3. Grabherr R., Strasser P., and VanEtten J. L. (1992). The DNA Polymerase Gene from Chlorella Viruses PBCV-1 and NY-2A Contains an Intron with Nuclear Splicing Sequences. Virology 188, 721-731.
  4. Nelson M., Zhang Y., Steffens D. L., Grabherr R., and VanEtten J. L. (1993). Sequencing Two DNA Templates in Five Channels by Digital Compression. Proc. Natl. Acad. Sci. USA 90, 1647-1651.
  5. Ernst W., Grabherr R.,and Katinger H. (1994).  Direct Cloning into the Autographa californica Nuclear Polyhedrosis Virus Genome for the Generation of Recombinant Baculovirus. Nucleic Acids Research 22, 2855-2856
  6. Engelhardt O., Grabherr R., Himmler G., and Rüker F. (1994). Two-step Cloning of Antibody Variable Domains in a Phage Display Vector. Biotechniques 17, 44-46.
  7. Zhang Y., Strasser P., Grabherr R., and VanEtten J. L. (1994).  Hairpin Loop Structure at the Termini of the Chlorella Virus PBCV-1 Genome. Virology 202, 1079-1082.
  8. Knezevic M., Vorauer-Uhl K., Hohenwarter O., Steindl F., Grabherr R. and Raspor P. (1996). Human Interleukin-2 (IL-2) Expressed by Transfected Mammalian Cells. E.  J. Physiol. 431, 227-228.
  9. Grabherr R., Ernst W., Dobblhof-Dier O., Sara, M. and Katinger H. (1997).  Expression of Foreign Proteins on the Surface of Autographa californica Nuclear Polyhedrosis Virus. Bio/Techniques 22: 730-735.
  10. Sowkas, S., Grabherr, R., Rüker, F., Plückthun, A., Scheiner , O., Breiteneder, H. (1997).  Chaperone-Assisted Expression of Allergen Peptide-Specific T-Cell Receptors.  J. Allergy and Clinical Immunology 99: 2020.
  11. Ernst, W., Grabherr, R., Wegner, D., Grassauer, A. and Katinger, H. W. D. (1998) Nucleic Acids Research 26: 1718-1723. Baculovirus Surface Display: Construction and Screening of a Eukaryotic Epitope Library.
  12. Ernst, W., Spenger, A., Toellner, L., Katinger H. and Grabherr R. (2000).  Expanding Baculovirus Surface Display: Modification of the Native Coat Protein gp64 of Autographa Californica NPV. Eur. J. Biochem. 167, 4033-4039.
  13. Grillari, J., Fortschegger, K., Grabherr, R., Hohenwarter O. and Katinger H. (2000) Analysis of Alterations in Gene Expression after Amplification of Recombinant Genes in CHO Cells. J. Biotechnol. 87, 59-65.
  14. Grillari, J., Hohenwarter O., Grabherr, R. and Katinger H. (2000) Subtractive Hybridization of mRNAs from Early and Senescent Endothelial Cells. Exp. Gerontology. 35. 187-197.
  15. Grillari, J., Hohenwarter, O., Grabherr, R. M., Katinger, H. (2001).  Isolation of differentially expressed genes in early passage and senescent human endothelial cells.Curr. Conc. Exp. Gerontology, Eds.: C. Bertoni-Freddari/H. Niedermüller, Facultas Wien. pp 111-123.
  16. Grabherr, R. and Ernst, W. (2001). The Baculovirus Expression System as a Tool for Generating Diversity by Viral Surface Display. Combinatorial Chemistry & High Throughput Screening 4, 190.
  17. Grabherr, R., Ernst, W., Oker-Blom, C. and Jones I. (2001). Developments in the use of baculoviruses for the surface display of complex eukaryotic proteins. TRENDS Biotechnol. 19, 231-23
  18. Striedner, G., Cserjan-Puschmann, M., Grabherr, R., Clementschitsch, Nilsson, E. and Bayer, K. (2001) Metabolic approaches to the optimisation of recombinant fermentation processes. Kluwer academic publishers, Recombinant protein production with prokaryotic and eukaryotic cells. A comparative view on host physiology. Editors: O. W. Merten, D. Mattanovich, C. Lang, G. Larsson, P. Neubauer, D. Posso, P. Postma, J. Teixeira deMattos, J. Cole.
  19. Grabherr, R. and Bayer K. (2001).  Vector Design Related to Bio-Processing. Food Technology and Biotechnology 39, 265-269
  20. Grabherr, R., Nilsson,E., Striedner, G. and Bayer, K. (2002).  Stabilising plasmid copy number to improve recombinant protein production.  Biotechnol. Bioeng. 77, 142-147.
  21. Grabherr, R. and Bayer K. (2002). Impact of targeted vector design on bacterial plasmid replication. TRENDS Biotechnol. 20, 257-260.
  22. Cserjan-Puschmann, M., Grabherr, R., Striedner, G., Clementschitsch, F. and Bayer K. (2002).  Integrated systems approach for the optimization of microbial recombinant fermentation processes. BioPharm 15, 26-34.
  23. Marzban, G., Grillari, J., Reisinger, E., Hemetsberger, T., Grabherr, R. and Katinger, H. (2002). Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries using proteomic tools. Exp. Gerontology 37, 1451-1460.
  24. Spenger, A., Grabherr, R., Töllner, L., Katinger, H. and Ernst, W. (2002) . Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64. Eur. J. Biochem. 269, 4458-4467.
  25. Grabherr, R. and Ernst, W. (2002)  Baculovirus major envelope protein gp64 and its application in eukaryotic surface display strategies. Recent Res. Devel. Biochem. 3, 445-457.
  26. Poomputsa, K., Egorov, A., Kittel, C., Ernst, W., Katinger, H. and Grabherr R. (2003).  Generation of recombinant influenza virus using baculovirus as a delivery vector. J. Vir. Methods 110, 111-114.
  27. Oker-Blom, C., Airenne, K. J. and Grabherr, R. (2003). Baculovirus surface display strategies: emerging tools for eukaryotic libraries and gene delivery Briefings in Funct. Genomics and Proteomics 2, p. 244-53.
  28. Ojala, K., Koski, J, Ernst, W., Grabherr, R., Jones, I. and Oker-Blom, C.(2004). Improved Display of Synthetic IgG-Binding Domains on the Baculovirus Surface. Technology in Cancer Research & Treatment, 3, 77-84
  29. Spenger, A., Ernst, W., Condreay,P., Kost,T. and Grabherr, R. (2004).Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells. Protein Expression and Purification 38, 17-23
  30. Fischlechner, M., Toellner, L., Messner, P., Grabherr, R., Donath, E. (2006) Virus-engineered colloidal particles-a surface display system. Angew. Chemie Int. Ed. 45, 784-789
  31. Toellner, L., Fischlechner, M., Ferko, B., Grabherr, R., Donath, E. (2006) Virus-coated LbL-colloids as a novel multiplex suspension array for the detection and quantification of virus-specific antibodies. Clinical Chemistry, in print.
  32. Matilainen, H., Riikonen, R., Saloniemi, T., Korhonen, E., Lehtinen, T., Hyypiä, T., Heino, J., Grabherr, R. and Oker-Blom, C. (2006). Presentation of RGD Motifs on the Surface of Baculovirus Enhances Viral Binding to Human Lung Carcinoma Cells. J. Biotechnology 125, 114-126 .
  33. Pfaffenzeller, I., Maihofer, J., Striedner, G., Bayer, K. and Grabherr, R. (2006). Using ColE1 derived RNA I for suppression of a bacterial encoded gene: Implication for a novel plasmid addiction system. Biotechnology Journal 1, 675-681.
  34. Ernst, W., Schinko, T., Spenger, A., Oker-Blom, C. and Grabherr, R. (2006) Improving baculovirus transduction of mammalian cells by surface display of a RGD-motif. J. Biotechnology, doi: 1016/j.jbiotec.2006.04.012
  35. Mäkelä, A., Ernst, W., Grabherr, R. and Oker-Blom, C. (2006) Baculovirus-based display and gene delivery systems. ColdSpringHarbor Laboratory Press, Gene Transfer: Delivery and Expression of DNA and RNA. Chapter 28.
  36. Nakowitsch, S., Kittel, C., Ernst, W., Egorov, A. and Grabherr, R. (2006) Optimization of baculovirus transduction on FreeStyleTM293 cells for the generation of influenza B/Lee/40. Molecular Biotechnology 34.
  37. Schinko, T., Thaisuchat, H., Viljoen, H., Padhye, N., Grabherr R. (2006) Fast and efficient generation of influenza A virus like particles from synthetic genes. Microbial Cell Factories 2006, 5 (Suppl 1): P39doi: 10.1186/1475-2859-5-S1-P39
  38. Mairhofer, J., Pfaffenzeller, I., Merz, D. and Grabherr, R. (2008) A novel antibiotic free plasmid selection system: advances in safe and efficient DNA therapy. Biotechnology Journal 3, 83-89. DOI: 10.1002/biot.200700141
  39. Mairhofer, J. and Grabherr, R. (2008) Rational vector design for efficient non-viral Gene Delivery: Challenges facing the use of plasmid DNA. Journal of Molecular Biotechnology (in press).
  40. Heinl, S., Hartinger, D., Thamhesl, M., Kunz-Vekiru, E., Krska, R., Schatzmayer, G., Moll, W.-D., Grabherr, R. (2010) Degradation of fumonisin B1 by the consecutive action of two bacterial enzymes . Journal of Biotechnology 145, 120-129.
  41. Krammer, F., Nakowitsch, S., Messner, P., Palmberger, D., Ferko, B., Grabherr. R. (2010) Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice.  Biotechnology Journal 5, 17-23.
  42. Mairhofer J, Cserjan-Puschmann M, Noebauer K, Razzazi-Fazeli E, Striedner G, Grabherr R. (2010) Marker free plasmids for gene therapeutic applications   lack of antibiotic resistance gene substantially improves the manufacturing process. Journal of Biotechnology.
  43. Krammer, F., Palmberger, D., Tauer, C., Messner, P., Grabherr, R. (2010) Trichoplusia ni cells (high-five) are highly efficient for the production of Influenza A virus-like particles; a comparison of two insect cell lines as production platforms for influenza vaccines. Molecular Biotechnology.
  44. Striedner, G., Pfaffenzeller, I., Luchner, M., Nemecek, S., Grabherr, R., Bayer Karl. (2010): Plasmid-free T7-based Escherichia coli expression systems. Biotechnol Bioeng. 105, 786-794.
  45. Heinl, S., Hartinger, D., Moll, W.-D. and Grabherr, R. (2010) Nucleotide sequence of a fumonisin B1 deaminating aminotransferase from the bacterium ATCC 55552. submitted for publication
  46. Krammer, F., Schinko, T., Messner, P., Palmberger, D., Ferko, B. and Grabherr, R. (2010) Presenting an ESAT-6 epitope: Influenza virus-like particles as antigen-carrier platform. submitted for publication. J VAC.
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